Question
Resit Assessment : Structure modelling
Outline: You will be given a sequence to model. You should search for a template and build models using alternative alignments. You will assess these using Ramachandran plots and further validation methods. You will research and briefly describe how the validation methods work.

1.Find a template to model this target sequence
>Rana
AGLTSLLSEKVKVFLMENKIHNFYQRELESVDSLIAIASRPVSDFKTIESMESSNSKQIRSIFKEAIQLLLKEGYIFQKDQNREVYQVTDQDKDL
You can find a file containing this sequence named Rana.txt on VITAL
Report→ (2 marks) Explain your choice of template, including a screenshot.

2.Using the following files make a model of the Rana protein
template_1WJ5.pdb
Rana_blast.ali
model_Rana_blast.py
The files are on VITAL in the same place as above.
Obtain an alternative alignment – target vs template - by using any one of the alignment programs in the table on the next page.

Make a second model.
Copy and modify the MODELLER files and make a model using your alternative alignment.
Report→ (2 marks) Copy and paste the contents of your modified files yourname.ali and yourname.py into your Word document.

3. Compare the Ramachandran plots of your two models.
Report→ (2 marks) Which model do you consider more reliable? Explain your answer and Include screenshots for both models.

4. For your chosen model, apply the ProSA and VERIFY_3D protein structure validation methods.
Report→ (5 marks) Including screenshots, describe what the results say about the quality of your chosen model
Using any resources you like, discover how the structure validation methods works.
Report→ (5 marks) Write a short description of each method in your report.

5. Thinking about different validation methods
Report→ (4 marks) With brief explanations, answer the following questions
- Do you think that a structure with a good ProSA score will always have a good Ramachandran plot?
- Do you think that a structure with a good Ramachandran plot will always score well by Verify_3D?
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Q. 1 Answer:
The Target sequence was allowed to aligned using the BLAST tool. The best hit for our query sequence was found to be 1WGN a CST sequence from the sequence. We picked the sequence as it shares maximum homology with the query sequence. The maximum percent of identity was 93.6% and thus was found to be the best template to model the structure of the protein. The results are shown in Figure 1. Figure 2 represents the Multiple sequence
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      • Illuminate\Cache\Events\CacheHit (199μs)
      • Illuminate\Cache\Events\CacheHit (262μs)
      • Illuminate\Cache\Events\CacheHit (286μs)
      • Illuminate\Cache\Events\CacheHit (217μs)
      • Illuminate\Cache\Events\CacheHit (169μs)
      • Illuminate\Cache\Events\CacheHit (191μs)
      • Illuminate\Cache\Events\CacheHit (169μs)
      • Illuminate\Cache\Events\CacheHit (190μs)
      • Illuminate\Cache\Events\CacheHit (168μs)
      • Illuminate\Cache\Events\CacheHit (232μs)
      • Illuminate\Cache\Events\CacheHit (210μs)
      • Illuminate\Cache\Events\CacheHit (205μs)
      • Illuminate\Cache\Events\CacheHit (179μs)
      • Illuminate\Cache\Events\CacheHit (194μs)
      • Illuminate\Cache\Events\CacheHit (169μs)
      • Illuminate\Cache\Events\CacheHit (188μs)
      • Illuminate\Cache\Events\CacheHit (170μs)
      • Illuminate\Cache\Events\CacheHit (206μs)
      • Illuminate\Cache\Events\CacheHit (171μs)
      • Illuminate\Cache\Events\CacheHit (196μs)
      • Illuminate\Cache\Events\CacheHit (158μs)
      • Illuminate\Cache\Events\CacheHit (272μs)
      • Illuminate\Cache\Events\CacheHit (267μs)
      • Illuminate\Cache\Events\CacheHit (193μs)
      • Illuminate\Cache\Events\CacheHit (157μs)
      • Illuminate\Cache\Events\CacheHit (184μs)
      • Illuminate\Cache\Events\CacheHit (157μs)
      • Illuminate\Cache\Events\CacheHit (181μs)
      • Illuminate\Cache\Events\CacheHit (166μs)
      • Illuminate\Cache\Events\CacheMissed (290μs)
      • Illuminate\Database\Events\StatementPrepared (940μs)
      • Illuminate\Database\Events\QueryExecuted (1.16ms)
      • eloquent.retrieved: App\Models\SubjectCat (64μs)
      • Illuminate\Cache\Events\KeyWritten (1.66ms)
      • Illuminate\Cache\Events\CacheHit (297μs)
      • Illuminate\Cache\Events\CacheHit (612μs)
      • Illuminate\Cache\Events\CacheHit (237μs)
      • Illuminate\Cache\Events\CacheHit (206μs)
      • Illuminate\Cache\Events\CacheHit (259μs)
      • Illuminate\Cache\Events\CacheHit (200μs)
      • Illuminate\Cache\Events\CacheHit (173μs)
      • Illuminate\Cache\Events\CacheHit (210μs)
      • Illuminate\Cache\Events\CacheHit (169μs)
      • Illuminate\Cache\Events\CacheHit (191μs)
      • Illuminate\Cache\Events\CacheHit (166μs)
      • Illuminate\Cache\Events\CacheHit (246μs)
      • Illuminate\Cache\Events\CacheHit (169μs)
      • Illuminate\Cache\Events\CacheHit (189μs)
      • Illuminate\Cache\Events\CacheHit (280μs)
      • Illuminate\Cache\Events\CacheHit (195μs)
      • Illuminate\Cache\Events\CacheHit (158μs)
      • creating: site.layouts.app (489μs)
      • composing: site.layouts.app (24μs)
      • creating: components.canonical (343μs)
      • composing: components.canonical (86μs)
      • creating: components.open-graph (150μs)
      • composing: components.open-graph (68μs)
      • creating: site.headers.header (324μs)
      • composing: site.headers.header (84μs)
      • Illuminate\Cache\Events\CacheHit (1.97ms)
      • creating: components.footer (98μs)
      • composing: components.footer (105μs)
      • Illuminate\Cache\Events\CacheHit (1.04ms)
      • Illuminate\Cache\Events\CacheMissed (414μs)
      • Illuminate\Database\Events\StatementPrepared (880μs)
      • Illuminate\Database\Events\QueryExecuted (1.2ms)
      • eloquent.retrieved: App\Models\SubjectCat (78μs)
      • Illuminate\Cache\Events\KeyWritten (404μs)
      • Illuminate\Cache\Events\CacheHit (329μs)
      • Illuminate\Cache\Events\CacheHit (155μs)
      • Illuminate\Cache\Events\CacheHit (479μs)
      • Illuminate\Cache\Events\CacheHit (254μs)
      • Illuminate\Cache\Events\CacheHit (215μs)
      • Illuminate\Cache\Events\CacheHit (317μs)
      • Illuminate\Cache\Events\CacheHit (395μs)
      • Illuminate\Cache\Events\CacheHit (247μs)
      • Illuminate\Cache\Events\CacheHit (211μs)
      • Illuminate\Cache\Events\CacheHit (181μs)
      • Illuminate\Cache\Events\CacheHit (204μs)
      • Illuminate\Cache\Events\CacheHit (252μs)
      • creating: components.forms.contact-us (318μs)
      • composing: components.forms.contact-us (197μs)
      • creating: components.forms.get-started (222μs)
      • composing: components.forms.get-started (92μs)
      • creating: components.forms.free-tool-download (159μs)
      • composing: components.forms.free-tool-download (79μs)
      • creating: components.forms.claim-free-worksheet (149μs)
      • composing: components.forms.claim-free-worksheet (74μs)
      • creating: components.forms.tutor-subscription-waitlist (139μs)
      • composing: components.forms.tutor-subscription-waitlist (67μs)
      • creating: components.forms.tutor-subscription-join (181μs)
      • composing: components.forms.tutor-subscription-join (73μs)
      • creating: components.forms.tutor-support (129μs)
      • composing: components.forms.tutor-support (66μs)
      • 311 x Illuminate\Cache\Events\CacheHit (16.07%)
        79.82ms
        20 x Illuminate\Database\Events\StatementPrepared (12.65%)
        62.83ms
        20 x Illuminate\Database\Events\QueryExecuted (6.91%)
        34.31ms
        11 x Illuminate\Cache\Events\KeyWritten (1.93%)
        9.59ms
        11 x Illuminate\Cache\Events\CacheMissed (1.45%)
        7.20ms
        1 x Illuminate\Foundation\Events\LocaleUpdated (0.89%)
        4.40ms
        12 x eloquent.retrieved: App\Models\SubjectCat (0.31%)
        1.55ms
        1 x Illuminate\Routing\Events\Routing (0.28%)
        1.39ms
        1 x Illuminate\Database\Events\ConnectionEstablished (0.16%)
        800μs
        1 x Illuminate\Routing\Events\RouteMatched (0.11%)
        540μs
        1 x creating: site.layouts.app (0.1%)
        489μs
        1 x creating: homework.show (0.09%)
        426μs
        1 x eloquent.booting: App\Models\SubjectCat (0.08%)
        400μs
        18 x eloquent.retrieved: App\Models\HomeworkLibrary\HomeworkLibraryFile (0.08%)
        377μs
        1 x creating: components.breadcrumbs (0.08%)
        374μs
        1 x creating: components.canonical (0.07%)
        343μs
        6 x eloquent.retrieved: App\Models\HomeworkLibrary\HomeworkLibrary (0.07%)
        338μs
        1 x creating: site.headers.header (0.07%)
        324μs
        1 x creating: components.forms.contact-us (0.06%)
        318μs
        1 x creating: components.forms.get-started (0.04%)
        222μs
        2 x eloquent.retrieved: App\Models\Subject (0.04%)
        209μs
        1 x composing: components.forms.contact-us (0.04%)
        197μs
        1 x creating: components.forms.tutor-subscription-join (0.04%)
        181μs
        1 x eloquent.booting: App\Models\Subject (0.04%)
        180μs
        1 x composing: components.breadcrumbs (0.04%)
        179μs
        1 x composing: homework.show (0.04%)
        176μs
        1 x eloquent.booting: App\Models\HomeworkLibrary\HomeworkLibrary (0.04%)
        174μs
        1 x eloquent.booted: App\Models\HomeworkLibrary\HomeworkLibrary (0.03%)
        165μs
        1 x creating: components.forms.free-tool-download (0.03%)
        159μs
        1 x creating: components.open-graph (0.03%)
        150μs
        1 x creating: components.forms.claim-free-worksheet (0.03%)
        149μs
        1 x creating: components.forms.tutor-subscription-waitlist (0.03%)
        139μs
        1 x creating: components.forms.tutor-support (0.03%)
        129μs
        1 x composing: components.footer (0.02%)
        105μs
        1 x creating: components.footer (0.02%)
        98μs
        1 x composing: components.forms.get-started (0.02%)
        92μs
        1 x composing: components.canonical (0.02%)
        86μs
        1 x composing: site.headers.header (0.02%)
        84μs
        1 x eloquent.booting: App\Models\HomeworkLibrary\HomeworkLibraryFile (0.02%)
        80μs
        1 x composing: components.forms.free-tool-download (0.02%)
        79μs
        1 x composing: components.forms.claim-free-worksheet (0.01%)
        74μs
        1 x composing: components.forms.tutor-subscription-join (0.01%)
        73μs
        1 x composing: components.open-graph (0.01%)
        68μs
        1 x composing: components.forms.tutor-subscription-waitlist (0.01%)
        67μs
        1 x composing: components.forms.tutor-support (0.01%)
        66μs
        1 x eloquent.booted: App\Models\Subject (0.01%)
        65μs
        1 x eloquent.booted: App\Models\SubjectCat (0.01%)
        52μs
        1 x eloquent.booted: App\Models\HomeworkLibrary\HomeworkLibraryFile (0.01%)
        37μs
        1 x composing: site.layouts.app (0%)
        24μs
      14 templates were rendered
      • 1x homework.showshow.blade.phpblade
      • 1x components.breadcrumbsbreadcrumbs.blade.phpblade
      • 1x site.layouts.appapp.blade.phpblade
      • 1x components.canonicalcanonical.blade.phpblade
      • 1x components.open-graphopen-graph.blade.phpblade
      • 1x site.headers.headerheader.blade.phpblade
      • 1x components.footerfooter.blade.phpblade
      • 1x components.forms.contact-uscontact-us.blade.phpblade
      • 1x components.forms.get-startedget-started.blade.phpblade
      • 1x components.forms.free-tool-downloadfree-tool-download.blade.phpblade
      • 1x components.forms.claim-free-worksheetclaim-free-worksheet.blade.phpblade
      • 1x components.forms.tutor-subscription-waitlisttutor-subscription-waitlist.blade.phpblade
      • 1x components.forms.tutor-subscription-jointutor-subscription-join.blade.phpblade
      • 1x components.forms.tutor-supporttutor-support.blade.phpblade
      uri
      GET college-homework-library/{category}/{subject}/{id}
      middleware
      web, utm.parameters
      controller
      App\Http\Controllers\HomeworkLibraryController@show
      namespace
      where
      as
      homework.show
      file
      app/Http/Controllers/HomeworkLibraryController.php:79-176
      20 statements were executed, 5 of which were duplicates, 15 unique. Show only duplicated70.98ms
      • Connection Establishedtwenty4_siteHomeworkLibraryController.php#91
        Backtrace
        • 13. app/Http/Controllers/HomeworkLibraryController.php:91
        • 14. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
        • 15. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
        • 16. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
        • 17. vendor/laravel/framework/src/Illuminate/Routing/Route.php:205
      • select * from `solutionslibrary` where `status` = 'published' and `price` > 0 and `solutionslibrary`.`id` = '50348' limit 1
        32.52mstwenty4_siteHomeworkLibraryController.php#97
        Bindings
        • 0: published
        • 1: 0
        • 2: 50348
        Backtrace
        • 16. app/Http/Controllers/HomeworkLibraryController.php:97
        • 17. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
        • 18. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
        • 19. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
        • 20. vendor/laravel/framework/src/Illuminate/Routing/Route.php:205
      • select * from `subjects` where `subjects`.`id` in (307)
        1.67mstwenty4_siteHomeworkLibraryController.php#97
        Backtrace
        • 21. app/Http/Controllers/HomeworkLibraryController.php:97
        • 22. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
        • 23. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
        • 24. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
        • 25. vendor/laravel/framework/src/Illuminate/Routing/Route.php:205
      • select * from `solutionslibrary_files` where `solutionslibrary_files`.`solutionlib_id` in (50348)
        870μstwenty4_siteHomeworkLibraryController.php#97
        Backtrace
        • 21. app/Http/Controllers/HomeworkLibraryController.php:97
        • 22. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
        • 23. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
        • 24. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
        • 25. vendor/laravel/framework/src/Illuminate/Routing/Route.php:205
      • select * from `subject_cats` where `subject_cats`.`id` = 3 limit 1
        1.02mstwenty4_siteHomeworkLibrary.php#201
        Bindings
        • 0: 3
        Backtrace
        • 20. app/Models/HomeworkLibrary/HomeworkLibrary.php:201
        • 26. app/Http/Controllers/HomeworkLibraryController.php:105
        • 27. vendor/laravel/framework/src/Illuminate/Routing/Controller.php:54
        • 28. vendor/laravel/framework/src/Illuminate/Routing/ControllerDispatcher.php:43
        • 29. vendor/laravel/framework/src/Illuminate/Routing/Route.php:260
      • select * from `solutionslibrary` where `id` <> 50348 and `subject` = 307 and `status` = 'published' and `price` > 0 order by RAND() limit 6
        9.25mstwenty4_siteHomeworkLibraryRepository.php#30
        Bindings
        • 0: 50348
        • 1: 307
        • 2: published
        • 3: 0
        Backtrace
        • 14. app/Repositories/HomeworkLibraryRepository.php:30
        • 15. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 16. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 18. app/Repositories/HomeworkLibraryRepository.php:39
        • 19. app/Http/Controllers/HomeworkLibraryController.php:139
      • select * from `subjects` where `subjects`.`id` in (307)
        1.39mstwenty4_siteHomeworkLibraryRepository.php#30
        Backtrace
        • 19. app/Repositories/HomeworkLibraryRepository.php:30
        • 20. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 21. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 23. app/Repositories/HomeworkLibraryRepository.php:39
        • 24. app/Http/Controllers/HomeworkLibraryController.php:139
      • select * from `solutionslibrary_files` where `solutionslibrary_files`.`solutionlib_id` = 50348 and `solutionslibrary_files`.`solutionlib_id` is not null and `publish` = 'question' order by `order` asc, `id` asc
        1.29mstwenty4_siteHomeworkLibrary.php#260
        Bindings
        • 0: 50348
        • 1: question
        Backtrace
        • 15. app/Models/HomeworkLibrary/HomeworkLibrary.php:260
        • 16. app/Transformers/HomeworkLibrary/HomeworkLibraryTransformer.php:58
        • 19. vendor/league/fractal/src/TransformerAbstract.php:128
        • 20. vendor/league/fractal/src/TransformerAbstract.php:107
        • 21. vendor/league/fractal/src/Scope.php:383
      • select * from `solutionslibrary_files` where `solutionslibrary_files`.`solutionlib_id` = 50348 and `solutionslibrary_files`.`solutionlib_id` is not null and `publish` = 'teaser' order by `order` asc, `id` asc
        1.33mstwenty4_siteHomeworkLibrary.php#260
        Bindings
        • 0: 50348
        • 1: teaser
        Backtrace
        • 15. app/Models/HomeworkLibrary/HomeworkLibrary.php:260
        • 16. app/Transformers/HomeworkLibrary/HomeworkLibraryTransformer.php:69
        • 19. vendor/league/fractal/src/TransformerAbstract.php:128
        • 20. vendor/league/fractal/src/TransformerAbstract.php:107
        • 21. vendor/league/fractal/src/Scope.php:383
      • select * from `solutionslibrary_files` where `solutionslibrary_files`.`solutionlib_id` = 50348 and `solutionslibrary_files`.`solutionlib_id` is not null and `publish` = 'solution' order by `order` asc, `id` asc
        1.15mstwenty4_siteHomeworkLibrary.php#260
        Bindings
        • 0: 50348
        • 1: solution
        Backtrace
        • 15. app/Models/HomeworkLibrary/HomeworkLibrary.php:260
        • 16. app/Transformers/HomeworkLibrary/HomeworkLibraryTransformer.php:80
        • 19. vendor/league/fractal/src/TransformerAbstract.php:128
        • 20. vendor/league/fractal/src/TransformerAbstract.php:107
        • 21. vendor/league/fractal/src/Scope.php:383
      • select * from `subject_cats` where `subject_cats`.`id` = 3 limit 1
        1.21mstwenty4_siteHomeworkLibrary.php#201
        Bindings
        • 0: 3
        Backtrace
        • 20. app/Models/HomeworkLibrary/HomeworkLibrary.php:201
        • 32. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
        • 33. vendor/laravel/framework/src/Illuminate/View/Engines/PhpEngine.php:58
        • 34. vendor/livewire/livewire/src/ComponentConcerns/RendersLivewireComponents.php:69
        • 35. vendor/laravel/framework/src/Illuminate/View/Engines/CompilerEngine.php:70
      • select * from `subject_cats` where `subject_cats`.`id` = 1 limit 1
        1.29mstwenty4_siteSubject.php#100
        Bindings
        • 0: 1
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 3 limit 1
        980μstwenty4_siteSubject.php#100
        Bindings
        • 0: 3
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 4 limit 1
        1.28mstwenty4_siteSubject.php#100
        Bindings
        • 0: 4
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 10 limit 1
        1.52mstwenty4_siteSubject.php#100
        Bindings
        • 0: 10
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 33 limit 1
        1.14mstwenty4_siteSubject.php#100
        Bindings
        • 0: 33
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 11 limit 1
        6.85mstwenty4_siteSubject.php#100
        Bindings
        • 0: 11
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 5 limit 1
        1.43mstwenty4_siteSubject.php#100
        Bindings
        • 0: 5
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 34 limit 1
        1.99mstwenty4_siteSubject.php#100
        Bindings
        • 0: 34
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 31 limit 1
        1.44mstwenty4_siteSubject.php#100
        Bindings
        • 0: 31
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 34. vendor/laravel/framework/src/Illuminate/Filesystem/Filesystem.php:110
      • select * from `subject_cats` where `subject_cats`.`id` = 36 limit 1
        1.36mstwenty4_siteSubject.php#100
        Bindings
        • 0: 36
        Backtrace
        • 18. app/Models/Subject.php:100
        • 19. vendor/laravel/framework/src/Illuminate/Cache/Repository.php:397
        • 20. vendor/laravel/framework/src/Illuminate/Cache/CacheManager.php:419
        • 22. app/Models/Subject.php:101
        • 28. view::components.footer:170
      App\Models\HomeworkLibrary\HomeworkLibraryFile
      18HomeworkLibraryFile.php
      App\Models\SubjectCat
      12SubjectCat.php
      App\Models\HomeworkLibrary\HomeworkLibrary
      6HomeworkLibrary.php
      App\Models\Subject
      2Subject.php
          _token
          vPsEXykcu2shRhPzlmuLvkGNR7ZLo7ogHav4YfeV
          utm_source
          direct
          redirectUrl
          /college-homework-library/Computer-Science/Bioinformatics/50348
          _previous
          array:1 [ "url" => "https://staging.dev.24houranswers.com/college-homework-library/Computer-Scienc...
          _flash
          array:2 [ "old" => [] "new" => [] ]
          PHPDEBUGBAR_STACK_DATA
          []
          path_info
          /college-homework-library/Computer-Science/Bioinformatics/50348
          status_code
          200
          
          status_text
          OK
          format
          html
          content_type
          text/html; charset=UTF-8
          request_query
          []
          
          request_request
          []
          
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